Hayashi K
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Genet Anal Tech Appl. 1992 Jun;9(3):73-9. doi: 10.1016/1050-3862(92)90001-l.
PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.
聚合酶链反应-单链构象多态性（PCR-SSCP）分析是基于PCR技术检测突变的最简单且可能最灵敏的方法之一。本文综述了PCR-SSCP的原理、实验指南及其在各个领域的应用。
