Suvorov Alexander N, Ferretti Joseph J
Institute of Experimental Medicine, Academy of Medical Sciences, Pavlova 12, St. Petersburg 197376, Russia.
J Basic Microbiol. 2004;44(1):66-74. doi: 10.1002/jobm.200310307.
A subtraction library of group B streptococcus (GBS) strain O9OR with GAS chromosomal DNA (strain SF370) was constructed and more than 100 plasmid clones sequenced. DNA sequences of the plasmid inserts were analyzed using the BLAST gene search. Most inserts had little or no homology to GAS chromosomal DNA and 26 clones from the library had no gene homologues in the gene bank. The majority of genes discovered represented house keeping GBS genes, but several could be considered as possible virulence factors. Inserts from 21 clones were labeled and used as probes for hybridization with GBS DNA fragments separated by pulsed field electrophoresis. A genetic map of GBS strain O9OR was constructed.
构建了B族链球菌（GBS）菌株O9OR与A组链球菌（GAS）染色体DNA（菌株SF370）的消减文库，并对100多个质粒克隆进行了测序。使用BLAST基因搜索对质粒插入片段的DNA序列进行了分析。大多数插入片段与GAS染色体DNA几乎没有同源性，文库中的26个克隆在基因库中没有基因同源物。发现的大多数基因代表GBS管家基因，但有几个基因可被视为可能的毒力因子。对21个克隆的插入片段进行标记，并用作与通过脉冲场电泳分离的GBS DNA片段杂交的探针。构建了GBS菌株O9OR的遗传图谱。
