Schillinger John, Du Vall Knorr Suzanne
J Environ Health. 2004 Jan-Feb;66(6):25-31, 43; quiz 45-6.
The study reported in this paper sampled 40 water vending machines distributed throughout the city of Los Angeles, California, in the water servicing area of the Los Angeles Department of Water and Power. The authors examined water samples for temperature, turbidity, chlorine content, fungal growth, coliform bacteria, fecal coliform bacteria, Pseudomonas spp., Pseudomonas aeruginosa, and heterotrophic plate counts using plate count agar and R2A agar. Roughly 48 percent of the original 40 samples were sampled in a second or third round, and these subsequent samples were examined for coliform bacteria, fecal coliform bacteria, Pseudomonas spp., Pseudomonas aeruginosa, and both heterotrophic plate counts. Observations were made of vending-machine external conditions, identifying information, and permits. Statistically significant associations were found between operator accessibility and poor machine conditions, operator accessibility and permits, and operator accessibility and the servicing interval. Statistically significant associations also were found between operator accessibility and presence of fungi, Pseudomonas spp., and Pseudomonas aeruginosa, and between presence of fungi and the servicing interval. In addition, statistically significant associations indicate that the quality of machine maintenance is a factor in microbial levels. The microbial content of the water suggested a need for further research into potential health effects in susceptible populations.
本文所报道的研究对分布在加利福尼亚州洛杉矶市、洛杉矶水电局供水服务区内的40台售水机进行了采样。作者使用平板计数琼脂和R2A琼脂对水样进行了温度、浊度、氯含量、真菌生长、大肠菌群、粪大肠菌群、假单胞菌属、铜绿假单胞菌以及异养平板计数的检测。最初的40个样本中约48%进行了第二轮或第三轮采样，后续样本检测了大肠菌群、粪大肠菌群、假单胞菌属、铜绿假单胞菌以及两种异养平板计数。对售水机的外部条件、识别信息和许可证进行了观察。在运营商可达性与机器状况不佳、运营商可达性与许可证、运营商可达性与维护间隔之间发现了具有统计学意义的关联。在运营商可达性与真菌、假单胞菌属和铜绿假单胞菌的存在之间，以及真菌的存在与维护间隔之间也发现了具有统计学意义的关联。此外，具有统计学意义的关联表明，机器维护质量是微生物水平的一个影响因素。水的微生物含量表明有必要进一步研究易感人群中潜在的健康影响。
