Tianiashin V I, Zimin V I, Boronin A M
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Mikrobiologiia. 2003 Nov-Dec;72(6):785-91.
The study of the cotransduction of the plasmid pairs pET-3a-pLysE and pET-3a-pLysS by the mutant phage T4alc7 showed that the antibiotic resistance markers of the plasmids were cotransduced with a high frequency. The analysis of the plasmid DNA of cotransductants and cotransformants showed that the mutant phage T4alc7 can be used for obtaining the monomeric and oligomeric forms of plasmids and for the cotransduction of two-plasmid overproduction systems into E. coli strains. The plaque mutants RB43-03 and RB43-13 derived from bacteriophage RB43 were found to be able to cotransduce the antibiotic resistance markers of pET-3a and pLysE plasmids.
对突变噬菌体T4alc7介导的质粒对pET - 3a - pLysE和pET - 3a - pLysS共转导的研究表明，质粒的抗生素抗性标记以高频共转导。对共转导子和共转化子的质粒DNA分析表明，突变噬菌体T4alc7可用于获得质粒的单体和寡聚体形式，以及将双质粒过量表达系统共转导到大肠杆菌菌株中。发现源自噬菌体RB43的噬菌斑突变体RB43 - 03和RB43 - 13能够共转导pET - 3a和pLysE质粒的抗生素抗性标记。
