San'kova T P, Svarchevskiĭ A N, Rybchin V N
Genetika. 1992 Jul;28(7):66-76.
Prophage of N15 temperate bacteriophage is stably maintained in Escherichia coli lysogens as a 46.33 kb linear plasmid. Using different transposons we obtained 18 insertion mutants of the N15 plasmid prophage. They were analysed for plaque formation ability, stability of the plasmid state and lysogenic conversion. Restriction mapping of the insertions allowed us to localize on the map the regions necessary for lytic growth and to map the lysogenic conversion gene. A recombinant phage encoding two antibiotic resistance genes was obtained. The phage contains an additional 4.77 kb DNA fragment (over 10% of the N15 genome).
N15温和噬菌体的原噬菌体作为一个46.33 kb的线性质粒稳定地维持在大肠杆菌溶原菌中。利用不同的转座子，我们获得了18个N15质粒原噬菌体的插入突变体。分析了它们的噬菌斑形成能力、质粒状态的稳定性和溶原性转化。插入片段的限制性图谱使我们能够在图谱上定位裂解生长所需的区域，并绘制溶原性转化基因的图谱。获得了一个编码两个抗生素抗性基因的重组噬菌体。该噬菌体含有一个额外的4.77 kb DNA片段（超过N15基因组的10%）。
