Blagodatskaia E V, Khokhlova O S, Anderson T H, Blagodatskiĭ S A
Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences, Pushchino, Moscow Region, Russia 142290.
Mikrobiologiia. 2003 Nov-Dec;72(6):847-53.
An evaluation of microbial DNA pools was performed using direct quantitative isolation of DNA from contemporary soils of Southern Urals and paleosols sealed under burial mounds of early Bronze Age more than 5000 years B.P. Significant regression dependence was found between the biomass and DNA contents in these soils (R2 = 0.97). Activity and dominant ecological strategies of microbial communities of paleosols and contemporary southern black soil were compared from growth parameters obtained by analysis of respiratory curves. The ratio of maximum specific growth rates of soil microorganisms on glucose and on yeast extract was shown to provide an auxotrophy index for soil microbial communities.
通过直接定量从南乌拉尔现代土壤以及距今5000多年前青铜时代早期古墓下封存的古土壤中分离DNA,对微生物DNA库进行了评估。发现这些土壤中的生物量与DNA含量之间存在显著的回归依赖性(R2 = 0.97)。根据呼吸曲线分析获得的生长参数,比较了古土壤和现代南方黑土微生物群落的活性和主要生态策略。结果表明,土壤微生物在葡萄糖和酵母提取物上的最大比生长速率之比可作为土壤微生物群落的营养缺陷型指数。