Lü Lin, Liu Shu-wen, Jiang Shi-bo, Wu Shu-guang
Institute of Pharmaceutical Sciences of the First Medical University of PLA, Key Lab of GD for Screening of New Drug, Guangzhou, Guangdong 510515, China.
Acta Pharmacol Sin. 2004 Feb;25(2):213-8.
To investigate the mechanism by which tannin inhibits HIV-1 entry into target cells.
The inhibitory activity of tannin on HIV-1 replication and entry was detected by p24 production and HIV-1-mediated cell fusion, respectively. The inhibitory activity on the gp41 six-helix bundle formation was determined by an improved sandwich ELISA.
Tannins from different sources showed potent inhibitory activity on HIV-1 replication, HIV-1-mediated cell fusion, and the gp41 six-helix bundle formation.
Tannin inhibits HIV-1 entry into target cells by interfering with the gp41 six-helix bundle formation, thus blocking HIV-1 fusion with the target cell.
研究单宁抑制HIV-1进入靶细胞的机制。
分别通过检测p24产生量和HIV-1介导的细胞融合，来检测单宁对HIV-1复制和进入的抑制活性。采用改进的夹心ELISA法测定其对gp41六螺旋束形成的抑制活性。
不同来源的单宁对HIV-1复制、HIV-1介导的细胞融合以及gp41六螺旋束形成均表现出强大的抑制活性。
单宁通过干扰gp41六螺旋束的形成来抑制HIV-1进入靶细胞，从而阻断HIV-1与靶细胞的融合。
