单宁通过靶向糖蛋白41抑制HIV-1进入。
Tannin inhibits HIV-1 entry by targeting gp41.
作者信息
Lü Lin, Liu Shu-wen, Jiang Shi-bo, Wu Shu-guang
机构信息
Institute of Pharmaceutical Sciences of the First Medical University of PLA, Key Lab of GD for Screening of New Drug, Guangzhou, Guangdong 510515, China.
出版信息
Acta Pharmacol Sin. 2004 Feb;25(2):213-8.
AIM
To investigate the mechanism by which tannin inhibits HIV-1 entry into target cells.
METHODS
The inhibitory activity of tannin on HIV-1 replication and entry was detected by p24 production and HIV-1-mediated cell fusion, respectively. The inhibitory activity on the gp41 six-helix bundle formation was determined by an improved sandwich ELISA.
RESULTS
Tannins from different sources showed potent inhibitory activity on HIV-1 replication, HIV-1-mediated cell fusion, and the gp41 six-helix bundle formation.
CONCLUSION
Tannin inhibits HIV-1 entry into target cells by interfering with the gp41 six-helix bundle formation, thus blocking HIV-1 fusion with the target cell.
目的
研究单宁抑制HIV-1进入靶细胞的机制。
方法
分别通过检测p24产生量和HIV-1介导的细胞融合，来检测单宁对HIV-1复制和进入的抑制活性。采用改进的夹心ELISA法测定其对gp41六螺旋束形成的抑制活性。
结果
不同来源的单宁对HIV-1复制、HIV-1介导的细胞融合以及gp41六螺旋束形成均表现出强大的抑制活性。
结论
单宁通过干扰gp41六螺旋束的形成来抑制HIV-1进入靶细胞，从而阻断HIV-1与靶细胞的融合。
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