Zhang Wei-zheng, Kaye David M
Wynn Department of Metabolic Cardiology, Baker Heart Research Institute, Melbourne Vic. 8008, Australia.
Anal Biochem. 2004 Mar 1;326(1):87-92. doi: 10.1016/j.ab.2003.11.006.
In an attempt to simultaneously detect molecules generated through the metabolism of l-arginine, a high-performance liquid chromatography method with on-line time-controlled preinjection reaction of ortho-phthaldialdehyde derivatization was developed. Plasma concentrations of citrulline, N(G)-hydroxy-l-arginine, N(G)-monomethyl-l-arginine, asymmetric N (G), N (G)-dimethyl-l-arginine, symmetric N (G), N (G')-dimethyl-l-arginine, ornithine, and agmatine were analyzed within 35min, using only 20microl of sample, pretreated by a simple cold ethanol cleanup procedure. Plasma samples of 35 healthy human volunteers were analyzed and results were comparable to other published data. All detection parameters of the method demonstrate that it is a reliable and efficient means for the comprehensive determination of arginine and its metabolites, making this approach suitable for routine clinical applications.
为了同时检测通过L-精氨酸代谢产生的分子，开发了一种具有邻苯二甲醛衍生化在线时间控制预注射反应的高效液相色谱方法。仅使用20微升样品，通过简单的冷乙醇净化程序进行预处理，在35分钟内分析了瓜氨酸、N（G）-羟基-L-精氨酸、N（G）-单甲基-L-精氨酸、不对称N（G），N（G）-二甲基-L-精氨酸、对称N（G），N（G'）-二甲基-L-精氨酸、鸟氨酸和胍丁胺的血浆浓度。分析了35名健康人类志愿者的血浆样本，结果与其他已发表的数据相当。该方法的所有检测参数表明，它是一种可靠且有效的手段，可用于全面测定精氨酸及其代谢物，使该方法适用于常规临床应用。
