Department of Central Laboratory, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
Clin Biochem. 2010 Sep;43(13-14):1141-7. doi: 10.1016/j.clinbiochem.2010.05.017. Epub 2010 Jun 9.
To develop a method for simultaneously determining l-citrulline and L-arginine levels in plasma using RP-HPLC with ultraviolet detection.
Plasma samples were deproteinized by trichloroacetic acid and heat. Phenyl-isothiocyanate (PITC) solution was used as derivatization reagent and a gradient elution was carried out.
The linearity for L-citrulline and L-arginine ranged from 0 to at least 1000 micromol/L. R(2) values were above 0.9999 for both. LODs for L-citrulline and L-arginine were 0.0201 micromol/L and 0.0476 micromol/L, respectively, while LOQs were 0.240 micromol/L and 0.448 micromol/L, respectively. Intra- and inter-day CVs were less than 3.40% and 7.2%, respectively. The average recovery was from 86.22% to 118.9%. L-citrulline and L-arginine concentrations in healthy controls were 60.77+/-9.18 micromol/L and 58.19+/-16.43 micromol/L, respectively.
This approach offers a reliable, efficient analytical platform for the simultaneous determination of citrulline and arginine levels in plasma.
建立一种同时测定血浆中 l-瓜氨酸和 L-精氨酸水平的反相高效液相色谱紫外检测法。
用三氯乙酸和热沉淀法处理血浆样品。苯异硫氰酸酯（PITC）溶液用作衍生化试剂，并进行梯度洗脱。
L-瓜氨酸和 L-精氨酸的线性范围均为 0 至至少 1000μmol/L。两者的 R(2)值均高于 0.9999。L-瓜氨酸和 L-精氨酸的检测限分别为 0.0201μmol/L 和 0.0476μmol/L，定量限分别为 0.240μmol/L 和 0.448μmol/L。日内和日间 CV 均小于 3.40%和 7.2%。平均回收率为 86.22%至 118.9%。健康对照组中 L-瓜氨酸和 L-精氨酸的浓度分别为 60.77+/-9.18μmol/L 和 58.19+/-16.43μmol/L。
该方法为同时测定血浆中瓜氨酸和精氨酸水平提供了可靠、高效的分析平台。
