Jin Kunlin, Mao Xiao Ou, Cottrell Barbara, Schilling Birgit, Xie Lin, Row Richard H, Sun Yunjuan, Peel Alyson, Childs Jocelyn, Gendeh Gurmil, Gibson Bradford W, Greenberg David A
Buck Institute for Age Research, Novato, California 94945, USA.
FASEB J. 2004 Feb;18(2):287-99. doi: 10.1096/fj.03-0973com.
Stathmin is a developmentally regulated cytosolic protein expressed at high levels in the brain. Two-dimensional differential in-gel electrophoresis and mass spectroscopy of proteins expressed in immature and mature cultures from embryonic rat cerebral cortex identified stathmin among several differentially expressed proteins, consistent with a possible role in neurogenesis. Stathmin immunohistochemistry in adult rodent brain revealed prominent expression in neuroproliferative zones and neuronal migration pathways, a pattern that resembles the expression of doublecortin, which is implicated in neuronal migration. Stathmin immunoreactivity was also associated with neurons undergoing ectopic chain migration into the ischemic striatum and cerebral cortex following focal cerebral ischemia. Reducing the expression of stathmin or doublecortin with an antisense oligonucleotide inhibited the migration of new neurons from the subventricular zone to the olfactory bulb via the rostral migratory stream. These results suggest a role for stathmin in the migration of newborn neurons in the adult brain.
Stathmin是一种在发育过程中受调控的胞质蛋白，在大脑中高水平表达。对来自胚胎大鼠大脑皮层的未成熟和成熟培养物中表达的蛋白质进行二维差异凝胶电泳和质谱分析，在几种差异表达的蛋白质中鉴定出了Stathmin，这与它在神经发生中可能发挥的作用一致。成年啮齿动物大脑中的Stathmin免疫组织化学显示，在神经增殖区和神经元迁移途径中有显著表达，这种模式类似于与神经元迁移有关的双皮质素的表达。在局灶性脑缺血后，Stathmin免疫反应性也与异位链式迁移到缺血纹状体和大脑皮层的神经元有关。用反义寡核苷酸降低Stathmin或双皮质素的表达，可抑制新神经元通过吻侧迁移流从脑室下区迁移到嗅球。这些结果表明Stathmin在成体大脑新生神经元的迁移中发挥作用。
