生存素的表达与具有生物学侵袭性的前列腺癌特征相关。

Survivin expression is associated with features of biologically aggressive prostate carcinoma.

作者信息

Shariat Shahrokh F, Lotan Yair, Saboorian Hossein, Khoddami Seyed M, Roehrborn Claus G, Slawin Kevin M, Ashfaq Raheela

机构信息

Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-91120, USA.

出版信息

Cancer. 2004 Feb 15;100(4):751-7. doi: 10.1002/cncr.20039.

DOI:10.1002/cncr.20039

PMID:14770431

Abstract

BACKGROUND

Survivin counteracts cell death and controls mitotic progression. The objectives of the current study were to compare the expression patterns of survivin in normal prostate, primary prostate carcinoma, and lymph node tissues involved with prostate carcinoma and to determine whether the expression of survivin is associated with prostate carcinoma characteristics and progression.

METHODS

Immunohistochemical staining for survivin and for transforming growth factor beta1 (TGF-beta1) and its receptors (types I and II; TGF-betaR1 and TGF-betaR2, respectively) was carried out on archival specimens from 114 consecutive patients who underwent radical prostatectomy (median follow-up, 64.8 months). Punch biopsies of the index carcinoma and normal tissue from each specimen were sectioned onto a single slide and stained. The authors also evaluated the expression of survivin in normal and malignant lymph node tissue from eight patients.

RESULTS

Survivin was expressed in 41 of 114 normal prostate specimens (36%) from prostates that contained carcinoma, in 81 of 114 primary prostate carcinoma specimens (71%), in 3 of 8 normal lymphoid specimens (38%), and in 7 of 8 prostate carcinoma lymphoid specimens (88%). Survivin expression was associated with higher final Gleason sum (P = 0.001), loss of TGF-betaR1 and TGF-betaR2 expression (P = 0.041 and P = 0.008, respectively), and an increased risk of biochemical progression on univariate analysis (P = 0.0441). Among patients who had disease progression, survivin was expressed more commonly in those who had tumors with features of aggressive behavior.

CONCLUSIONS

The expression of survivin gradually increased from normal prostate tissue, to low-grade primary carcinoma, to high-grade primary carcinoma and was highest in lymph node metastases. Survivin expression was associated further with alteration of the TGF-beta pathway and with overall and aggressive biochemical progression after radical prostatectomy.

摘要

背景

Survivin可抵抗细胞死亡并控制有丝分裂进程。本研究的目的是比较Survivin在正常前列腺组织、原发性前列腺癌组织以及前列腺癌累及的淋巴结组织中的表达模式，并确定Survivin的表达是否与前列腺癌的特征及进展相关。

方法

对114例接受根治性前列腺切除术的连续患者（中位随访时间为64.8个月）的存档标本进行Survivin、转化生长因子β1（TGF-β1）及其受体（I型和II型，分别为TGF-βR1和TGF-βR2）的免疫组化染色。将每个标本的肿瘤活检组织和正常组织的穿刺活检标本切片置于同一张载玻片上进行染色。作者还评估了8例患者正常和恶性淋巴结组织中Survivin的表达。

结果

在114例含有癌的前列腺组织的正常前列腺标本中，41例（36%）表达Survivin；在114例原发性前列腺癌标本中，81例（71%）表达Survivin；在8例正常淋巴组织标本中，3例（38%）表达Survivin；在8例前列腺癌淋巴结标本中，7例（88%）表达Survivin。Survivin的表达与较高的最终Gleason评分（P = 0.001）、TGF-βR1和TGF-βR2表达缺失（分别为P = 0.041和P = 0.008）以及单因素分析中生化进展风险增加相关（P = 0.0441）。在疾病进展的患者中，Survivin在具有侵袭性行为特征肿瘤的患者中更常见。