Koshlukova S E, Markovska T T, Pankov R G, Momchilova A B, Koumanov K S
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Biochimie. 1992 Nov;74(11):981-7. doi: 10.1016/0300-9084(92)90018-a.
Investigations have been carried out on the alterations of membrane lipids and some enzyme activities during liver regeneration. The results indicated that 32 h after partial hepatectomy the membrane phospholipids per mg protein were augmented. The cholesterol esters were also increased in both microsomal and plasma membranes. The specific radioactivity of the separate phospholipid fractions, estimated by incorporation of 14C-palmitate into the phospholipid molecules, was higher in membranes from partially hepatectomized rats, compared to sham-operated ones, indicating an enhanced phospholipid synthesis. The content and specific radioactivity of diacylglycerols and triacylglycerols was enhanced in both types of membranes from regenerating liver. Moreover, we observed a fluidization of these membranes, which is illustrated by the decrease of the structural order parameter (SDPH) of the lipid bilayer as well as by the elevation of the excimer to monomer fluorescent ratio (IE/IM). 1,6-Diphenyl-1,3,5-hexatriene and pyrene were used as fluorescent probes for determination of the membranes physical state. Palmitoyl-CoA and oleoyl-CoA synthetase, acyl-CoA: lysophosphocholine and acyl-CoA:lysophosphoethanolamine acyltransferase as well as phospholipase C activities were augmented in membranes from partially hepatectomized rats. The biological significance of these alterations in the process of liver regeneration is discussed.
对肝脏再生过程中膜脂和一些酶活性的变化进行了研究。结果表明，部分肝切除术后32小时，每毫克蛋白质的膜磷脂增加。微粒体膜和质膜中的胆固醇酯也增加。通过将14C-棕榈酸酯掺入磷脂分子来估计的各磷脂组分的比放射性，与假手术组相比，部分肝切除大鼠的膜中更高，表明磷脂合成增强。再生肝脏的两种类型膜中二酰甘油和三酰甘油的含量和比放射性均增加。此外，我们观察到这些膜的流化，这通过脂质双层结构序参数（SDPH）的降低以及准分子与单体荧光比率（IE/IM）的升高来体现。1,6-二苯基-1,3,5-己三烯和芘用作荧光探针来测定膜的物理状态。部分肝切除大鼠的膜中棕榈酰辅酶A和油酰辅酶A合成酶、酰基辅酶A：溶血卵磷脂和酰基辅酶A：溶血磷脂乙醇胺酰基转移酶以及磷脂酶C活性增加。讨论了这些变化在肝脏再生过程中的生物学意义。
