视紫红质和细菌视紫红质的基质辅助激光解吸质谱分析
Matrix-assisted laser desorption mass spectrometry of rhodopsin and bacteriorhodopsin.
作者信息
Schey K L, Papac D I, Knapp D R, Crouch R K
机构信息
Department of Pharmacology, Medical University of South Carolina, Charleston 29425.
出版信息
Biophys J. 1992 Nov;63(5):1240-3. doi: 10.1016/S0006-3495(92)81699-5.
Abstract
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry has been used to obtain accurate molecular weight information for the integral membrane proteins bacteriorhodopsin and bovine rhodopsin desorbed from solubilized membrane preparations. Mass differences in the molecular weights measured for bleached and unbleached bacteriorhodopsin and rhodopsin indicate the removal of the retinal chromophores upon bleaching. The MALDI technique was also successful for determination of the major cleavage products obtained upon treatment of membrane bound rhodopsin with endoproteinase Asp-N and thermolysin. Our results indicate that the MALDI method is a useful means of obtaining accurate molecular weight information on hydrophobic proteins isolated in their native membranes.
摘要
基质辅助激光解吸电离（MALDI）质谱已被用于从溶解的膜制剂中解吸的完整膜蛋白细菌视紫红质和牛视紫红质获取准确的分子量信息。对漂白和未漂白的细菌视紫红质和视紫红质测量的分子量质量差异表明漂白时视黄醛发色团被去除。MALDI技术也成功用于测定用天冬氨酸内肽酶-N和嗜热菌蛋白酶处理膜结合视紫红质时获得的主要裂解产物。我们的结果表明，MALDI方法是获得关于在其天然膜中分离的疏水蛋白准确分子量信息的有用手段。
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