Bordoni A, Biagi P L, Turchetto E, Rossi C A, Hrelia S
Department of Biochemistry G Moruzzi, University of Bologna, Italy.
Cardioscience. 1992 Dec;3(4):251-5.
Using cultures of beating neonatal rat cardiomyocytes we have studied the fatty acid composition of the diacylglycerol produced after different stimulation times with an alpha 1-agonist (phenylephrine) and we have related it to the previously reported time course of the activation of particulate protein kinase C, in control cells and in cells grown in a medium supplemented with docosahexaenoic acid. Gas chromatography of the diacylglycerol produced after stimulation revealed significant differences between control cells and cells treated with docosahexanoic acid. In the cells treated with docosahexanoic acid, the more persistent activation of the membrane-bound protein kinase C might be sustained by an enrichment of diacylglycerol with docosahexanoic acid. The modification of the fatty acid composition of diacylglycerol can cause an alteration in the response of the cells to alpha 1-adrenoceptor stimulation.
我们使用新生大鼠搏动心肌细胞培养物，研究了用α1-激动剂（去氧肾上腺素）刺激不同时间后产生的二酰基甘油的脂肪酸组成，并将其与先前报道的在对照细胞和在添加二十二碳六烯酸的培养基中生长的细胞中颗粒状蛋白激酶C激活的时间进程相关联。刺激后产生的二酰基甘油的气相色谱分析显示对照细胞和用二十二碳六烯酸处理的细胞之间存在显著差异。在用二十二碳六烯酸处理的细胞中，膜结合蛋白激酶C更持久的激活可能由富含二十二碳六烯酸的二酰基甘油维持。二酰基甘油脂肪酸组成的改变可导致细胞对α1-肾上腺素能受体刺激的反应发生改变。
