Alexander D, Stinson J, Pear J, Glascock C, Ward E, Goodman R M, Ryals J
Calgene, Inc., Davis, CA 95616.
Mol Plant Microbe Interact. 1992 Nov-Dec;5(6):513-5. doi: 10.1094/mpmi-5-513.
A previously undescribed cDNA family was isolated from tobacco challenged with tobacco mosaic virus (TMV). A cDNA library was constructed with mRNA from upper leaves of Xanthi nc tobacco plants that had been inoculated with TMV on the lower leaves 11 days previously. The library was screened differentially with radiolabeled cDNA synthesized with mRNA from upper, uninoculated leaves of either TMV-inoculated or mock-inoculated tobacco plants. The new cDNA family, designated SAR8.2, had at least five expressed members, one or more of which were inducible by TMV inoculation and by salicylic acid treatment. The cDNAs encoded small, highly basic proteins containing N-terminal hydrophobic signal peptides and highly conserved cysteine-rich C-terminal domains. One of the SAR8.2 family members contained a direct repeat of the C-terminal domain in tandem. Hybridization of SAR8.2 cDNA to tobacco genomic DNAs indicated a gene family of 10-12 members.
从受烟草花叶病毒（TMV）侵染的烟草中分离出一个此前未被描述的cDNA家族。用11天前在下部叶片接种了TMV的Xanthi nc烟草植株上部叶片的mRNA构建了一个cDNA文库。用从TMV接种或模拟接种的烟草植株上部未接种叶片的mRNA合成的放射性标记cDNA对该文库进行差异筛选。这个新的cDNA家族被命名为SAR8.2，至少有五个表达成员，其中一个或多个成员可被TMV接种和水杨酸处理诱导。这些cDNA编码小的、高度碱性的蛋白质，含有N端疏水信号肽和高度保守的富含半胱氨酸的C端结构域。SAR8.2家族成员之一包含C端结构域的串联直接重复序列。SAR8.2 cDNA与烟草基因组DNA的杂交表明这是一个由10-12个成员组成的基因家族。
