Naqvi S M, Park K S, Yi S Y, Lee H W, Bok S H, Choi D
Plant Protectants Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, South Korea.
Plant Mol Biol. 1998 Jun;37(3):571-6. doi: 10.1023/a:1006031316476.
During efforts for cloning disease resistance-responsive genes, a cDNA encoding a putative Nicotiana glutinosa glycine-rich RNA binding protein (ngRBP) was isolated from TMV induced cDNA library. Northern blot hybridization revealed that ngRBP gene is negatively regulated during early hours of TMV induced acute hypersensitive response (HR). Under greenhouse conditions induced expression of ngRBP gene was observed after 24 h following TMV infection. Salicylic acid and copper also induced ngRBP mRNA expression. Our findings are suggestive of some possible role for ngRBP in plant-pathogen interaction.
在克隆抗病反应基因的过程中，从烟草花叶病毒（TMV）诱导的cDNA文库中分离出一个编码假定的粘毛烟草富含甘氨酸RNA结合蛋白（ngRBP）的cDNA。Northern杂交分析表明，在TMV诱导的急性过敏反应（HR）早期，ngRBP基因受到负调控。在温室条件下，TMV感染24小时后观察到ngRBP基因的诱导表达。水杨酸和铜也能诱导ngRBP mRNA的表达。我们的研究结果表明ngRBP在植物与病原体的相互作用中可能发挥某些作用。
