Cacciafesta M, Ferri C, Scuteri A, Piccirillo G, Bellucci C, Persichino L, Ariani A, Marigliano V, Delfini M, Miccheli A
Institute of I Clinica Medica, Policlinico Umberto I, Roma, Italy.
Cell Mol Biol (Noisy-le-grand). 1992 Dec;38(8):877-84.
The behaviour of sodium transport systems across the cell membrane has been poorly investigated in elderly hypertension. Sodium efflux driven by Na+/K+/Cl-cotransport activity was therefore investigated (using a novel NMR-spectroscopy method) in 5 elderly hypertensive males (mean age 78 +/- 5 years) and 5 normotensive controls (mean age 79 +/- 3 years). In order to exclude any change in cotransport activity secondary to metabolic abnormalities, both patients and controls were non-obese and had normal glucose and lipid metabolisms. The Na+/K+/Cl-cotransport evaluation was performed after three months of pharmacological wash-out, under a diet containing 120 mEq of Na+/day. The resulting data showed that Na+ efflux due to outward Na+/K+/Cl-cotransport was higher in hypertensive group than in the normotensive one (0.50 +/- 0.10 mmol Na+/l cells/hr. vs 0.33 +/- 0.03 mmol Na+/l cells/hr., respectively, p < 0.05). Intracellular Na+ content was similar in both groups. At variance with previous data from the literature, our findings indicate that the Na+/K+/Cl-cotransport activity is elevated in elderly hypertensives.
在老年高血压患者中，细胞膜上钠转运系统的行为尚未得到充分研究。因此，我们（采用一种新的核磁共振波谱法）对5名老年男性高血压患者（平均年龄78±5岁）和5名血压正常的对照者（平均年龄79±3岁）的由Na+/K+/Cl协同转运活性驱动的钠外流进行了研究。为了排除继发于代谢异常的协同转运活性的任何变化，患者和对照者均非肥胖，且葡萄糖和脂质代谢正常。在经过三个月的药物洗脱期后，在每日摄入120 mEq Na+的饮食条件下进行Na+/K+/Cl协同转运评估。所得数据显示，高血压组由外向Na+/K+/Cl协同转运引起的Na+外流高于血压正常组（分别为0.50±0.10 mmol Na+/l细胞/小时和0.33±0.03 mmol Na+/l细胞/小时，p<0.05）。两组细胞内Na+含量相似。与先前文献数据不同的是，我们的研究结果表明老年高血压患者的Na+/K+/Cl协同转运活性升高。
