Borson N D, Salo W L, Drewes L R
Department of Biochemistry and Molecular Biology, School of Medicine, University of Minnesota, Duluth 55812.
PCR Methods Appl. 1992 Nov;2(2):144-8. doi: 10.1101/gr.2.2.144.
We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.
