Garcia M M, Becker S A, Brooks B W, Berg J N, Finegold S M
Agriculture Canada, Animal Diseases Research Institute, Nepean, Ontario.
Can J Vet Res. 1992 Oct;56(4):318-25.
The ultrastructural features and molecular components of 18 strains of Fusobacterium necrophorum biovars A, AB and B, isolated from animal and human infections, were examined by electron microscopy, multilocus enzyme electrophoresis (MEE) and by sodium dodecyl sulfate-gradient polyacrylamide gel electrophoresis (SDS-PAGE). High resolution scanning electron microscopy revealed that the strains possessed a convoluted surface pattern. Transmission electron microscopy showed that all strains possessed a cell wall structure typical of gram-negative bacteria. Bleb formation was not uncommon. Numerous extracellular materials, resembling lipopolysaccharide (LPS) fragments, surrounded cells of both human strains and biovar B animal strains. Biovar A field strains revealed capsules as stained by ruthenium red whereas a stock culture strain showed the capsule only when immunostabilized with hyperimmune serum. Starch gel electrophoresis showed all strains to possess adenyl kinase, glutamate dehydrogenases and lactate dehydrogenase; each enzyme migrated uniformly (monomorphic) among the strains and represented an electrotype. However, SDS-PAGE indicated differences in the protein profiles between all of the strains; the most distinctly different was a human isolate (FN 606). Silver staining to detect LPS showed extensive "ladder" patterns among the majority of biovar A strains but not in the animal biovar B strains. Immunoblotting of LPS with a rabbit antiserum prepared against phenol extracted LPS from a biovar A animal isolate (LA 19) suggested marked variability in the LPS antigens among the isolates studied.
对从动物和人类感染中分离出的18株坏死梭杆菌生物变种A、AB和B的超微结构特征和分子成分，通过电子显微镜、多位点酶电泳（MEE）以及十二烷基硫酸钠梯度聚丙烯酰胺凝胶电泳（SDS-PAGE）进行了检测。高分辨率扫描电子显微镜显示，这些菌株具有卷曲的表面模式。透射电子显微镜表明，所有菌株都具有典型的革兰氏阴性菌细胞壁结构。形成泡状结构并不罕见。大量类似脂多糖（LPS）片段的细胞外物质围绕着人类菌株和生物变种B动物菌株的细胞。生物变种A的现场菌株经钌红染色显示有荚膜，而一株保藏培养菌株仅在用超免疫血清进行免疫固定时才显示出荚膜。淀粉凝胶电泳显示所有菌株都具有腺苷激酶、谷氨酸脱氢酶和乳酸脱氢酶；每种酶在菌株间迁移一致（单态），代表一种电型。然而，SDS-PAGE表明所有菌株之间的蛋白质谱存在差异；差异最明显的是一株人类分离株（FN 606）。用于检测LPS的银染色显示，大多数生物变种A菌株中有广泛的“梯状”模式，而动物生物变种B菌株中则没有。用针对从生物变种A动物分离株（LA 19）中酚提取的LPS制备的兔抗血清对LPS进行免疫印迹分析表明，在所研究的分离株中LPS抗原存在明显差异。
