Bermúdez V M, Miller R B, Rosendal S, Fernando M A, Johnson W H, O'Brien P J
Department of Pathology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1992 Oct;56(4):331-8.
The cytopathic effects induced by five strains of Mycoplasma equigenitalium for cells of equine uterine tube explants were tested by measuring changes in cellular and extracellular concentrations of calmodulin (CaM). Calmodulin concentrations in samples of total homogenate (TH) and total homogenate supernates (THS) of the infected equine uterine tube explants were significantly lower than respective measurements on noninfected controls. In tissue culture medium fractions (TCM) of some infected explants, CaM concentrations were significantly higher than noninfected controls (p > 0.95). The results suggest that M. equigenitalium colonization on ciliated cells of the equine uterine tube can affect the permeability of the cell membrane leading to leakage or release of CaM during cell breakdown. Measurement of CaM concentrations in samples of TH revealed significant differences in the cytotoxic effects induced by different strains of M. equigenitalium on the equine uterine tube (EUT). The data suggests that some strains of M. equigenitalium may have a role in reproductive failure in the mare. In addition comparisons of the means of the concentrations of CaM in samples of TH or THS in EUT explants from four mares in the follicular and four in the luteal phase of the estrous cycle were found to be not significantly different.
通过测量钙调蛋白（CaM）在细胞内和细胞外浓度的变化，测试了五株马生殖道支原体对马输卵管外植体细胞的细胞病变效应。感染马输卵管外植体的总匀浆（TH）和总匀浆上清液（THS）样本中的钙调蛋白浓度显著低于未感染对照组的相应测量值。在一些感染外植体的组织培养基组分（TCM）中，CaM浓度显著高于未感染对照组（p>0.95）。结果表明，马生殖道支原体在马输卵管纤毛细胞上的定殖可影响细胞膜的通透性，导致细胞破裂期间CaM泄漏或释放。对TH样本中CaM浓度的测量揭示了不同株马生殖道支原体对马输卵管（EUT）诱导的细胞毒性效应存在显著差异。数据表明，某些马生殖道支原体菌株可能在母马繁殖失败中起作用。此外，发现在发情周期的卵泡期和黄体期，四匹母马的EUT外植体中TH或THS样本中CaM浓度平均值的比较无显著差异。
