Smits B, Rosendal S, Ruhnke H L, Plante C, O'Brien P J, Miller R B
Department of Pathology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1994 Apr;58(2):114-21.
Calmodulin (CAM) acts as an intracellular regulator of calcium, an important mediator of many cell processes. We used the CAM assay and electron microscopy to investigate the effects of Ureaplasma diversum on bovine oviductal explants obtained aseptically from slaughtered cows. A stock suspension of U. diversum (treated specimens) and sterile broth (controls) was added to replicates of cultured explants and incubated at 38 degrees C in an atmosphere of 5.5% CO2 for 48 hours. Explants were examined for ciliary activity, extracellular CAM loss, and for histological and ultrastructural changes. Explants and their culture media were examined for changes in CAM concentration. All experiments were replicated three times. In addition, U. diversum, medium and broth were assayed for CAM content. The concentrations of CAM in explants and media changed significantly (p < 0.05) in samples which were inoculated with U. diversum when compared to controls. The controls and infected specimens did not differ histologically or ultrastructurally, but U. diversum was seen to be closely associated with infected explant tissue. In view of this close affinity it is assumed the loss of CAM from the oviductal cells was causally related, but this was not proven. The failure to show cell membrane injury on light and electron microscopic examination was probably related to the short duration of the experiment and may only point out the sensitivity of the CAM assay in detecting early cell membrane injury. Compromise in characteristics of the medium to support both, the viability of oviductal cells and U. diversum limited the experimental time to 48 hours.(ABSTRACT TRUNCATED AT 250 WORDS)
钙调蛋白（CAM）作为细胞内钙的调节剂，是许多细胞过程的重要介质。我们使用CAM检测法和电子显微镜来研究不同种脲原体对从屠宰母牛无菌获取的牛输卵管外植体的影响。将不同种脲原体的储备悬液（处理样本）和无菌肉汤（对照）添加到培养外植体的重复样本中，并在38摄氏度、5.5%二氧化碳的气氛中孵育48小时。检查外植体的纤毛活性、细胞外CAM损失以及组织学和超微结构变化。检查外植体及其培养基中CAM浓度的变化。所有实验重复三次。此外，检测不同种脲原体、培养基和肉汤中的CAM含量。与对照相比，接种不同种脲原体的样本中外植体和培养基中CAM的浓度发生了显著变化（p < 0.05）。对照和感染样本在组织学或超微结构上没有差异，但可以看到不同种脲原体与感染的外植体组织密切相关。鉴于这种密切的亲和力，推测输卵管细胞中CAM的损失存在因果关系，但这尚未得到证实。在光学和电子显微镜检查中未能显示细胞膜损伤可能与实验持续时间短有关，并且可能仅表明CAM检测法在检测早期细胞膜损伤方面的敏感性。培养基在支持输卵管细胞活力和不同种脲原体生长特性方面的不足将实验时间限制在48小时。（摘要截断于250字）
