Klamut H J, Kotarba J A, Strickland K P
Muscle Nerve. 1987 Jan;10(1):69-76. doi: 10.1002/mus.880100113.
Calmodulin levels have been assessed in whole muscle and primary culture extracts in order to examine the relationship between calmodulin and the accumulation of calcium in dystrophic hamster muscle tissues. Significant decreases in both normal and dystrophic skeletal muscle, tongue, and heart calmodulin levels were observed between 2 and 12 weeks of age. Dystrophic values, however, tended to be somewhat higher than normal, especially in 12-week-old skeletal muscle total and soluble extracts (normal 29.7 and 0.6 and dystrophic 117.0 and 3.1 micrograms/g wet weight, respectively). No significant differences were observed in dystrophic myoblast (total 2.22-2.78, soluble 2.85-3.26 micrograms/mg protein) or fibroblast (total 2.64-2.94, soluble 2.54-3.60 micrograms/mg protein) calmodulin levels, except for a significant decrease in dystrophic fibroblast levels (total 1.97, soluble 2.18 micrograms/mg protein) at 7 days in culture. Elevated calmodulin levels in dystrophic muscle are discussed in terms of increases in intracellular Ca2+ concentrations and immature regenerating fibers.
为了研究钙调蛋白与营养不良仓鼠肌肉组织中钙积累之间的关系，已对全肌肉和原代培养提取物中的钙调蛋白水平进行了评估。在2至12周龄之间，观察到正常和营养不良的骨骼肌、舌头及心脏中的钙调蛋白水平均显著下降。然而，营养不良组的值往往略高于正常组，尤其是在12周龄的骨骼肌总提取物和可溶性提取物中（正常组分别为29.7和0.6微克/克湿重，营养不良组分别为117.0和3.1微克/克湿重）。除了培养7天时营养不良的成纤维细胞水平显著下降（总钙调蛋白为1.97微克/毫克蛋白，可溶性钙调蛋白为2.18微克/毫克蛋白）外，在营养不良的成肌细胞（总钙调蛋白为2.22 - 2.78微克/毫克蛋白，可溶性钙调蛋白为2.85 - 3.26微克/毫克蛋白）或成纤维细胞（总钙调蛋白为2.64 - 2.94微克/毫克蛋白，可溶性钙调蛋白为2.54 - 3.60微克/毫克蛋白）的钙调蛋白水平上未观察到显著差异。从细胞内Ca2+浓度增加和未成熟再生纤维的角度讨论了营养不良肌肉中钙调蛋白水平升高的问题。
