Oliw E H
Department of Pharmaceutical Pharmacology, Uppsala University Biomedical Centre, Sweden.
J Chromatogr. 1992 Dec 2;583(2):231-5. doi: 10.1016/0378-4347(92)80557-7.
cis-Epoxides of linoleic acid, alpha-linolenic acid, arachidonic acid and the omega 3-epoxide of eicosapentaenoic acid were chromatographed on a cellulose trisphenylcarbamate (Chiralcel OC) stationary phase in the normal-phase mode. The R,S and S,R enantiomers of methyl-14(15)epoxyeicosatrienoate, methyl-9(10)epoxyoctadecadienoate and methyl-9(10)epoxyoctadecenoate could be partly resolved. The R,S enantiomer of methyl-14(15)epoxyeicosatrienoate eluted before the S,R enantiomer. [14C]14(15)Epoxyeicosatrienoic acid was isolated from an incubation of [14C]20:4n-6 with microsomes of rabbit kidney cortex and the S,R enantiomer was found to predominate (about 2:1).
亚油酸、α-亚麻酸、花生四烯酸的顺式环氧化物以及二十碳五烯酸的ω-3环氧化物在纤维素三苯基氨基甲酸酯（手性拆分柱Chiralcel OC）固定相上以正相模式进行色谱分析。14(15)-环氧二十碳三烯酸甲酯、9(10)-环氧十八碳二烯酸甲酯和9(10)-环氧十八碳烯酸甲酯的R,S和S,R对映体能够得到部分分离。14(15)-环氧二十碳三烯酸甲酯的R,S对映体比S,R对映体先洗脱出来。从[14C]20:4n-6与兔肾皮质微粒体的孵育物中分离出了[14C]14(15)-环氧二十碳三烯酸，发现其中S,R对映体占主导（约为2:1）。
