Andersen C, Balmér K, Lagerstróm P O
Astra Hässle AB, Mölndal, Sweden.
J Chromatogr. 1993 May 19;615(1):159-63. doi: 10.1016/0378-4347(93)80303-l.
A stereoselective method for the two enantiomers of warfarin has been developed. Warfarin is extracted into dichloromethane-hexane from blood plasma. After concentration by evaporation, the enantiomers are resolved by liquid chromatography on Chiralcel OC employing a non-polar mobile phase. By fluorometric detection, concentrations in plasma down to 80 nmol/l (25 ng/ml) can be determined with a coefficient of variation of less than 15%. At the 200 nmol/l level, a coefficient of variation of ca. 4% was found.
已开发出一种用于华法林两种对映体的立体选择性方法。华法林从血浆中萃取到二氯甲烷 - 己烷中。蒸发浓缩后，对映体在手性纤维素OC上通过液相色谱法分离，采用非极性流动相。通过荧光检测，血浆中低至80 nmol/l（25 ng/ml）的浓度可被测定，变异系数小于15%。在200 nmol/l水平时，发现变异系数约为4%。
