Grizard G, Lombard-Vignon N, Boucher D
Service de Biologie du Développement et de la Reproduction, CHRU 30 Clermont-Ferrand, France.
Hum Reprod. 1992 Oct;7(9):1245-8. doi: 10.1093/oxfordjournals.humrep.a137835.
L-Carnitine and acetylcarnitine concentrations were determined in spermatozoa and seminal plasma from 15 men, in both fresh ejaculate and frozen-thawed semen with cryoprotective medium. Sperm motility was also evaluated. In fresh samples, the levels of carnitine and acetylcarnitine in seminal plasma were comparable whereas in spermatozoa, acetylcarnitine predominated. Cryopreservation did not change the carnitine and acetylcarnitine levels in seminal plasma nor the carnitine concentration in spermatozoa; by contrast, the acetylcarnitine level in spermatozoa was decreased in 14 cases (110 +/- 8 versus 210 +/- 20 nmol/10(8) cells). This decrease in acetylcarnitine content was greater during semen dilution in cryoprotectant than after the freezing/thawing process. Motility was also decreased in all cases after the freezing/thawing process. These results suggest that acetylcarnitine recovery in spermatozoa is further evidence of the deleterious effect of the cryoprotective medium in the cryopreservation of semen.
测定了15名男性新鲜精液及添加冷冻保护剂的冻融精液中精子和精浆中左旋肉碱及乙酰肉碱的浓度，并评估了精子活力。在新鲜样本中，精浆中肉碱和乙酰肉碱水平相当，而在精子中，乙酰肉碱占主导。冷冻保存未改变精浆中肉碱和乙酰肉碱水平，也未改变精子中肉碱浓度；相比之下，14例精子中乙酰肉碱水平降低（从210±20降至110±8 nmol/10⁸细胞）。在精液用冷冻保护剂稀释过程中，乙酰肉碱含量的降低比冷冻/解冻过程后更大。冷冻/解冻后所有病例的精子活力也均降低。这些结果表明，精子中乙酰肉碱的减少进一步证明了冷冻保护剂在精液冷冻保存中具有有害作用。
