Suter D A, Holland M K
Fertil Steril. 1979 May;31(5):541-4. doi: 10.1016/s0015-0282(16)44001-x.
Free-L-carnitine and L-O-acetylcarnitine concentrations have been determined in spermatozoa and seminal plasma of normal, fresh, and frozen human semen. Results show that in fresh semen most of the free L-carnitine (0.213 +/- 0.02 mM) and L-O-acetylcarnitine (0.063 +/- 0.007 mM) is found in the seminal plasma. In contrast to other worker's results, the concentrations of free L-carnitine and L-O-acetylcarnitine in spermatozoa were found to be high and were 0.384 +/- 0.066 mumole/10(9) spermatozoa (22 mM) and 0.376 +/- 0.057 mumole/10(9) spermatozoa (21.6 mM), respectively. It is also demonstrated that the distribution of soluble metabolites such as L-carnitine between spermatozoa and seminal plasma is altered by the freezing of semen. After freezing and storage of semen at -20 degrees C for 7 days, the intracellular concentrations of free L-carnitine and L-O-acetylcarnitine decreased to below the limits of assay.
已测定正常、新鲜及冷冻人精液的精子和精浆中游离左旋肉碱和左旋-O-乙酰肉碱的浓度。结果表明，在新鲜精液中，大部分游离左旋肉碱（0.213±0.02 mM）和左旋-O-乙酰肉碱（0.063±0.007 mM）存在于精浆中。与其他研究者的结果相反，精子中游离左旋肉碱和左旋-O-乙酰肉碱的浓度较高，分别为0.384±0.066微摩尔/10⁹个精子（22 mM）和0.376±0.057微摩尔/10⁹个精子（21.6 mM）。研究还表明，精液冷冻会改变精子和精浆之间诸如左旋肉碱等可溶性代谢物的分布。精液在-20℃冷冻保存7天后，游离左旋肉碱和左旋-O-乙酰肉碱的细胞内浓度降至检测限以下。
