Tartof D, Fitch F W
J Immunol. 1978 Apr;120(4):1086-91.
Induction of maximal CTL activity was achieved within 12 hr of exposure to Con A in vitro in various mouse lymphoid cell populations. These included spleen cells from normal unsensitized mice, spleen cells from mice previously immunized with alloantigen, and mouse spleen cells exposed to alloantigen in long-term mixed leukocyte culture (LTMLC). Although induction of maximal incorporation of tritiated thymidine was accomplished within this same period in the cells obtained from LTMLC, a much longer period of Con A exposure (greater than 24 hr) was required for freshly prepared spleen cells from normal or previously immunized mice. These findings indicate that the increased tritiated thymidine uptake induced in freshly prepared spleen cells on continued exposure to Con A beyond 12 hr is not associated with the development of cytolytic activity, and that it probably represents stimulation of subpopulations no longer present in the LTMLC population where positive selection for cells responsive to cellular alloantigens has taken place.
在体外，将各种小鼠淋巴细胞群体暴露于刀豆蛋白A（Con A）12小时内可诱导出最大的细胞毒性T淋巴细胞（CTL）活性。这些群体包括来自正常未致敏小鼠的脾细胞、先前用同种异体抗原免疫的小鼠的脾细胞，以及在长期混合淋巴细胞培养（LTMLC）中暴露于同种异体抗原的小鼠脾细胞。虽然在LTMLC获得的细胞中，在同一时期内实现了最大程度的氚化胸腺嘧啶核苷掺入，但来自正常或先前免疫小鼠的新鲜制备的脾细胞需要更长时间的Con A暴露（超过24小时）。这些发现表明，新鲜制备的脾细胞在持续暴露于Con A超过12小时后诱导的氚化胸腺嘧啶核苷摄取增加与细胞溶解活性的发展无关，并且它可能代表了对LTMLC群体中不再存在的亚群的刺激，在LTMLC群体中已经对响应细胞同种异体抗原的细胞进行了阳性选择。
