Peavy D L, Pierce C W
J Immunol. 1975 Dec;115(6):1521-4.
The role of cellular proliferation in the development of cytotoxic lymphocyte (CL) responses in one-way mixed lymphocyte reactions was investigated by using tritiated thymidine of high specific activity to kill proliferating cells. To develop maximum CL responses, responding lymphoid cells must proliferate for approximately 72 hr; thereafter, precursors of CL appear to differentiate into active CL without further proliferation. Different alloantigen-sensitive precursor cell populations participate in the CL responses to each of two sets of stimulating alloantigens. When cells responding to one set of alloantigens were selectively destroyed after incorporating the hot thymidine, the surviving cells retained the capacity to develop a normal CL response to the second set of alloantigens.
通过使用高比活度的氚化胸腺嘧啶核苷来杀伤增殖细胞，研究了细胞增殖在单向混合淋巴细胞反应中细胞毒性淋巴细胞（CL）应答发育过程中的作用。为了产生最大的CL应答，反应性淋巴细胞必须增殖约72小时；此后，CL前体细胞似乎分化为活性CL而无需进一步增殖。不同的同种异体抗原敏感前体细胞群体参与对两组刺激同种异体抗原中每一组的CL应答。当对一组同种异体抗原作出反应的细胞在掺入热胸腺嘧啶核苷后被选择性破坏时，存活的细胞保留了对第二组同种异体抗原产生正常CL应答的能力。
