Loud A V, Anversa P, Giacomelli F, Wiener J
Lab Invest. 1978 May;38(5):586-96.
A method for determining the mean absolute volume of a specific population of cells within a tissue is described and applied to the measurement of endocardial and epicardial myocytes in the left ventricle of normal and hypertensive rats. The technique, based on nuclear counts per unit area in tissue slices of different known thicknesses, measures the mean cell volume per nucleus independent of previously unknown nuclear dimensions and systematic counting errors. Duplicate determinations, demonstrating reproducibility, were made in mutually perpendicular longitudinal and transverse sections of the myocardium. Combining these light microscopic measurements with electron microscopic data enabled the evaluation of the mean diameter and length of the cylindrical myocyte nuclei showing those in the epicardial cells to be significantly longer than the nuclei in endocardial cells. It was estimated that 2 to 4 per cent of ventricular myocytes are binucleate. After 1 to 4 weeks of hypertension, induced by constriction of the left renal artery, endocardial myocytes were enlarged 21 per cent, from 10,370 +/- 410 to 12,520 +/- 490 cu. micrometer., while epicardial myocytes showed a 37 per cent hypertrophy, from 12,600 +/- 1,600 to 17,300 +/- 1,100 cu. micrometer. The availability of a reliable determination of cell volume will make possible the interpretation of much biochemical, functional, and morphometric data at the whole cell level.
本文描述了一种确定组织内特定细胞群体平均绝对体积的方法，并将其应用于正常和高血压大鼠左心室内心内膜和心外膜心肌细胞的测量。该技术基于不同已知厚度组织切片中的单位面积核计数，可测量每个核的平均细胞体积，而不受先前未知的核尺寸和系统计数误差的影响。在心肌的相互垂直的纵向和横向切片中进行了重复测定，证明了可重复性。将这些光学显微镜测量结果与电子显微镜数据相结合，能够评估圆柱形心肌细胞核的平均直径和长度，结果显示心外膜细胞中的核明显长于心内膜细胞中的核。据估计，2%至4%的心室肌细胞为双核。在通过左肾动脉缩窄诱导高血压1至4周后，心内膜心肌细胞增大了21%，从10370±410立方微米增加到12520±490立方微米，而心外膜心肌细胞显示出37%的肥大，从12600±1600立方微米增加到17300±1100立方微米。可靠的细胞体积测定方法的可用性将使在全细胞水平上解释许多生化、功能和形态测量数据成为可能。
