Kyle K M, Harauz G
Department of Molecular Biology and Genetics, College of Biological Science, University of Guelph, Ontario, Canada.
Mol Cell Biochem. 1992 Nov 4;117(1):11-21. doi: 10.1007/BF00230406.
The complex comprising 5S ribosomal RNA and the ribosomal protein YL3 (5S rRNP) was isolated from yeast (Saccharomyces cerevisiae), and positively contrasted preparations were imaged by transmission electron microscopy. The overall dimensions of the 5S rRNP complex in the micrographs were 10 nm by 6 nm. Three predominant projections were selected from several hundred putative particles for digitisation and computer averaging to yield two-dimensional constructions with reproducible spatial resolutions exceeding 2 nm. The enhanced projection images were compatible with structural models of this complex based on biochemical studies.
从酵母（酿酒酵母）中分离出由5S核糖体RNA和核糖体蛋白YL3组成的复合物（5S核糖体核糖核蛋白），并通过透射电子显微镜对正反差制剂进行成像。显微照片中5S核糖体核糖核蛋白复合物的整体尺寸为10纳米×6纳米。从数百个假定的颗粒中选择了三个主要投影进行数字化和计算机平均，以产生空间分辨率超过2纳米的二维结构。增强后的投影图像与基于生化研究的该复合物结构模型相符。
