Jones C M, Simon-Chazottes D, Guenet J L, Hogan B L
Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232.
Mol Endocrinol. 1992 Nov;6(11):1961-8. doi: 10.1210/mend.6.11.1480182.
A cDNA clone, Vgr-2, with homology to certain members of the transforming growth factor-beta superfamily has been isolated from a mouse embryo cDNA library. The encoded protein shows significant similarity to members of the Vg-1/decapentaplegic/bone morphogenetic protein subgroup of the transforming growth factor-beta family. Within this group, Vgr-2 is more similar to Xenopus Vg-1 than to any other member so far isolated. The gene is expressed at highest levels during midgestation mouse development, and transcripts are localized by in situ hybridization to the osteogenic zone of developing bone. Vgr-2 is expressed in F9 teratocarcinoma cells, and its RNA levels are down-regulated within 24 h after differentiation with retinoic acid. The genomic organization of Vgr-2 and its location on mouse chromosome 6 are reported.
从一个小鼠胚胎cDNA文库中分离出了一个与转化生长因子-β超家族的某些成员具有同源性的cDNA克隆Vgr-2。编码的蛋白质与转化生长因子-β家族的Vg-1/果蝇的decapentaplegic/骨形态发生蛋白亚组的成员显示出显著的相似性。在这个亚组中，Vgr-2与非洲爪蟾的Vg-1比与迄今分离出的任何其他成员更为相似。该基因在小鼠妊娠中期发育期间表达水平最高，通过原位杂交将转录本定位到发育中骨骼的成骨区。Vgr-2在F9畸胎癌细胞中表达，在用视黄酸分化后24小时内其RNA水平下调。报道了Vgr-2的基因组结构及其在小鼠6号染色体上的位置。
