Wang C, Chen T, Xia K, Li Y, Liu Y, Yang D, Yin M
Wuhan Institute of Virology, Academia Sinica.
Wei Sheng Wu Xue Bao. 1992 Oct;32(5):383-6.
A cockroachcidal bacterial isolate CW-W-90-3 was selected by egg yolk agar plate. The isolate produced phospholipase C (PLC) which was pathogenic to the nymph of cockroach. The conditions for production of high level PLC indicated that using LB medium supplement Tween-80 or minimal medium could effectively increase the activity of PLC. The optical phase for production of PLC was in the period of 12-18 hours and below pH8.0. The activity of PLC was reduced along with the culture time until 48 hours. The PLC was resistant to heat. The partially purified PLC from the culture supernatant was assayed by using cockroach nymphes and produced 71.74% mortality.
通过蛋黄琼脂平板筛选出一种具有杀蟑螂活性的细菌菌株CW-W-90-3。该菌株产生对蟑螂若虫具有致病性的磷脂酶C（PLC）。高水平PLC的产生条件表明，使用添加吐温80的LB培养基或基本培养基可有效提高PLC的活性。产生PLC的最佳光学阶段是在12至18小时期间且pH值低于8.0。直到48小时，PLC的活性随着培养时间而降低。PLC耐热。用蟑螂若虫对培养上清液中部分纯化的PLC进行测定，死亡率达71.74%。
