Ravasi Pablo, Braia Mauricio, Eberhardt Florencia, Elena Claudia, Cerminati Sebastián, Peirú Salvador, Castelli Maria Eugenia, Menzella Hugo G
Genetic Engineering & Fermentation Technology, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario-IPROBYQ-Conicet, Suipacha 531, Rosario 2000, Argentina.
Keclon, S.A. Tucuman 7180, Rosario 2000, Argentina.
J Biotechnol. 2015 Dec 20;216:142-8. doi: 10.1016/j.jbiotec.2015.10.018. Epub 2015 Oct 28.
Enzymatic oil degumming (removal of phospholipids) using phospholipase C (PLC) is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the production of recombinant Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5g/L of the recombinant enzyme was achieved when the respective gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzed phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a manufacturing process for the cost effective production of this enzyme.
使用磷脂酶C（PLC）进行酶法脱胶（去除磷脂）是一种成熟且环保的植物油精炼工艺。在本研究中，我们报道了在谷氨酸棒杆菌ATCC 13869中通过高细胞密度发酵生产重组蜡样芽孢杆菌PLC及其在大豆油脱胶中的性能。当在半限定培养基中从tac启动子表达相应基因时，重组酶的最终浓度达到5.5g/L。用胰蛋白酶处理以切割前肽后，成熟酶完全水解了占大豆油中磷脂70%的磷脂酰胆碱和磷脂酰乙醇胺。此处给出的结果表明了使用蜡样芽孢杆菌PLC进行油脱胶的可行性，并提供了一种经济高效生产该酶的制造工艺。
