Vanderschueren D, Van Herck E, Suiker A M, Allewaert K, Visser W J, Geusens P, Bouillon R
Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Katholieke Universiteit Leuven, Belgium.
J Bone Miner Res. 1992 Dec;7(12):1407-15. doi: 10.1002/jbmr.5650071208.
The effects of androgen and estrogen deficiency on skeletal homeostasis were studied in the guinea pig. Male and female adult (7 months old) guinea pigs were either sham operated (9 females and 7 males) or gonadectomized [9 ovariectomized (OVX) females and 6 orchidectomized (ORX) males] and sacrificed 4 months later for evaluation of bone mass, bone turnover, and serum calcium homeostasis. Parameters of bone turnover, calcium homeostasis, and vitamin D metabolites were similar in all groups except for increased serum IGF-I concentrations (+30%) in males compared to females. Gonadectomy resulted in a 50% decrease in serum IGF-I concentrations in males only (p < 0.001). Volume, total calcium content, and cortical density of the tibia were significant higher in males than in females. Estrogen deficiency had no effect on bone volume or calcium content. Androgen deficiency resulted in a significant lower volume and calcium content of the tibia and in a lower calcium content of the distal lumbar vertebrae. Single-photon absorptiometry of the tibia showed that only cortical, not trabecular bone density of the tibia was decreased after ORX. Histomorphometric studies of the tibial metaphysis also did not show significant differences in trabecular bone volume between sham-operated and ORX males. We conclude that in adult male guinea pigs androgen deficiency results in a decrease in (cortical) bone volume and content concomitant with decreased IGF-I levels. In female guinea pigs of the same age, estrogen deficiency did not affect total or regional bone mass.
在豚鼠中研究了雄激素和雌激素缺乏对骨骼稳态的影响。成年（7个月大）雄性和雌性豚鼠分别接受假手术（9只雌性和7只雄性）或去势手术[9只卵巢切除（OVX）雌性和6只睾丸切除（ORX）雄性]，4个月后处死以评估骨量、骨转换和血清钙稳态。除雄性血清IGF-I浓度比雌性增加30%外，所有组的骨转换、钙稳态和维生素D代谢产物参数相似。去势仅导致雄性血清IGF-I浓度降低50%（p<0.001）。雄性胫骨的体积、总钙含量和皮质密度显著高于雌性。雌激素缺乏对骨体积或钙含量没有影响。雄激素缺乏导致胫骨体积和钙含量显著降低，以及腰椎远端钙含量降低。胫骨单光子吸收测定显示，ORX后仅胫骨皮质骨密度降低，小梁骨密度未降低。胫骨干骺端的组织形态计量学研究也未显示假手术和ORX雄性之间小梁骨体积有显著差异。我们得出结论，在成年雄性豚鼠中，雄激素缺乏导致（皮质）骨体积和含量减少，同时IGF-I水平降低。在同年龄的雌性豚鼠中，雌激素缺乏不影响总体或局部骨量。
