Makler A, Makler-Shiran E, Stoller J, Lissak A, Abramovici H, Blumenfeld Z
Department of Ob-Gyn, Rambam Medical Center and Carmel Hospital, Technion Faculty of Medicine, Haifa, Israel.
Arch Androl. 1992 Nov-Dec;29(3):255-61. doi: 10.3109/01485019208987733.
To study the correlation between metabolism and motility, ejaculated human spermatozoa were washed in media containing glucose, pyruvate, and deoxyglucose in various combinations. Spermatozoa suspended in these media were incubated in sealed mini-chambers and subjected to aerobic or anaerobic conditions at 37 degrees C. The effect on the patterns of sperm motility was investigated in real time by direct observation and objective determination with the multiple exposure photography (MEP) method. The motility of spermatozoa incubated in media containing excess of glucose showed similar changes of motility quality with time, whether exposed to aerobic or anaerobic conditions, and in both cases motility lasted about 13 h. Motility of sperm incubated with pyruvate only was of a much lower quality, especially under anaerobic conditions, and in both circumstances lasted about 7 h. When glycolysis of fructose remnants was totally inhibited by deoxyglucose and sperm were incubated with pyruvate only, motility lasted for 2 h under aerobic conditions and only for about 1 h under anaerobic conditions. It is concluded that the main metabolic process that supplies energy for sperm motility is glycolysis, under both aerobic and anaerobic conditions. Oxidative respiration was less efficient as a source of energy for sperm motility. When glycolysis was inhibited and oxidative respiration was eliminated under anaerobic conditions, sperm motility lasted only for about 1 h, probably by using intracellular energy reserves.
为了研究代谢与活力之间的相关性，将射出的人类精子在含有葡萄糖、丙酮酸和脱氧葡萄糖的各种组合的培养基中洗涤。悬浮在这些培养基中的精子在密封的小室中孵育，并在37℃下进行有氧或无氧条件处理。通过直接观察和用多次曝光摄影（MEP）方法进行客观测定，实时研究对精子活力模式的影响。在含有过量葡萄糖的培养基中孵育的精子，无论暴露于有氧还是无氧条件下，其活力质量随时间的变化相似，在两种情况下活力持续约13小时。仅与丙酮酸孵育的精子活力质量要低得多，尤其是在无氧条件下，在两种情况下都持续约7小时。当果糖残余物的糖酵解被脱氧葡萄糖完全抑制且精子仅与丙酮酸孵育时，在有氧条件下活力持续2小时，在无氧条件下仅持续约1小时。得出的结论是，在有氧和无氧条件下，为精子活力提供能量的主要代谢过程是糖酵解。氧化呼吸作为精子活力的能量来源效率较低。在无氧条件下，当糖酵解被抑制且氧化呼吸被消除时，精子活力仅持续约1小时，可能是利用细胞内的能量储备。
