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火鸡(吐绶鸡)精子在有氧或无氧、有葡萄糖或果糖情况下的代谢与活力

Metabolism and motility of turkey (Meleagris gallopavo) spermatozoa in the presence or absence of oxygen, glucose and fructose.

作者信息

Amir D, Pinto O, Schindler H, Hurwitz S

出版信息

Comp Biochem Physiol A Comp Physiol. 1985;80(3):325-7. doi: 10.1016/0300-9629(85)90044-1.

DOI:10.1016/0300-9629(85)90044-1
PMID:2858309
Abstract

Sugar consumption by turkey spermatozoa for 1 hr at 37 degrees C was similar in the presence or absence of oxygen, and with glucose or fructose in the medium. Motility of the spermatozoa at the end of the above incubation period was lower under anaerobic than aerobic conditions. Fructose enhanced the oxygen uptake of spermatozoa in comparison with that in the glucose-containing medium. Omission of sugar from the medium depressed respiration but not motility of the spermatozoa. The rate of oxygen uptake by the spermatozoa during a 3-hr incubation period was higher in a 3.3-mM than a 15.0-mM glucose medium. Fructose was formed from glucose under aerobic but not under anaerobic conditions. Fructose originating from glucose was used for fructolysis, when the glucose reserve in the medium was almost exhausted.

摘要

在37摄氏度下,火鸡精子在有氧或无氧条件下,以及培养基中含有葡萄糖或果糖时,1小时的糖消耗量相似。在上述孵育期结束时,精子的活力在厌氧条件下低于需氧条件。与含葡萄糖的培养基相比,果糖增强了精子的氧气摄取。培养基中不含糖会抑制呼吸,但不会抑制精子的活力。在3小时的孵育期内,精子在3.3毫摩尔葡萄糖培养基中的氧气摄取率高于15.0毫摩尔葡萄糖培养基。在有氧而非无氧条件下,葡萄糖可形成果糖。当培养基中的葡萄糖储备几乎耗尽时,由葡萄糖产生的果糖用于糖酵解。

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