Gautron J P, Pattou E, Kordon C
Unité INSERM 159 de Dynamique des Systèmes Neuroendocriniens Centre Paul Broca, Paris, France.
Biochem Biophys Res Commun. 1992 Dec 30;189(3):1368-73. doi: 10.1016/0006-291x(92)90225-a.
A naturally occurring analog of the decapeptide luteinizing hormone-releasing hormone ([Hyp9]LHRH) has been described previously in the hypothalamus of several mammals. It derives from post-translational hydroxylation of the LHRH proline9 residue. In the present work, intermediate LHRH precursors exhibiting both Pro9 or Hyp9 residues in the LHRH sequence were characterized in the rat hypothalamus. Hydroxylation of the Pro9 residue can thus be assumed to occur at an early stage of post-translational maturation. Deaminated, free acid forms of both native decapeptides were also detected. They correspond most likely to catabolites from incompletely processed precursors.
一种天然存在的十肽促黄体生成激素释放激素类似物（[Hyp9]LHRH）此前已在几种哺乳动物的下丘脑被发现。它源自LHRH脯氨酸9残基的翻译后羟基化。在本研究中，对大鼠下丘脑内LHRH序列中同时含有Pro9或Hyp9残基的中间LHRH前体进行了表征。因此可以假定脯氨酸9残基的羟基化发生在翻译后成熟的早期阶段。还检测到了两种天然十肽的脱氨基游离酸形式。它们很可能对应于未完全加工前体的分解代谢产物。
