人类下丘脑促黄体激素释放激素前体与大鼠和小鼠下丘脑的促黄体激素释放激素前体大小相同。

The human hypothalamic LHRH precursor is the same size as that in rat and mouse hypothalamus.

作者信息

Curtis A, Lyons V, Fink G

出版信息

Biochem Biophys Res Commun. 1983 Dec 28;117(3):872-7. doi: 10.1016/0006-291x(83)91677-7.

Abstract

The synthesis of the decapeptide luteinizing hormone releasing hormone (LHRH) in human, rat and mouse brain has been investigated by studying the in vitro translation products of Poly A+ mRNA extracts from the hypothalamus. The translation products of all three species contained a single 28000 MW polypeptide which immunoprecipitated with a specific anti-LHRH serum. This polypeptide was not present in the translation products of Poly A+ mRNA extracts from the hypothalamus of the hypogonadal mouse, a mutant strain totally deficient in LHRH. These results show that in the human, rat and normal mouse, LHRH is synthesized as a component of a precursor peptide with a molecular weight of 28000.

摘要

通过研究来自下丘脑的聚腺苷酸（Poly A+）信使核糖核酸（mRNA）提取物的体外翻译产物，对人、大鼠和小鼠脑中十肽促黄体生成激素释放激素（LHRH）的合成进行了研究。所有这三个物种的翻译产物都包含一种单一的28000道尔顿（MW）的多肽，该多肽能与特异性抗LHRH血清发生免疫沉淀反应。这种多肽不存在于性腺功能减退小鼠下丘脑的聚腺苷酸（Poly A+）信使核糖核酸（mRNA）提取物的翻译产物中，该突变株完全缺乏LHRH。这些结果表明，在人、大鼠和正常小鼠中，LHRH是作为一种分子量为28000的前体肽的组成部分而合成的。