Murphy C T, Peers S H, Forder R A, Flower R J, Carey F, Westwick J
Department of Pharmacology, University of Bath, Avon, UK.
Biochem Biophys Res Commun. 1992 Dec 30;189(3):1739-46. doi: 10.1016/0006-291x(92)90279-t.
In this study the identity of annexins in human platelets has been determined together with their ability to be released by agents which induce platelet degranulation. The presence of proteins cross-reacting to antibodies against annexins I and V was detected in human platelets. However, the study provided evidence that these annexins are not located on the surface of the plasma membrane in a Ca++ dependent manner. Moreover, activation of platelets with several agents which induced platelet degranulation did not cause release of annexins I or V as determined by both immunoblotting and ELISA.
在本研究中，已确定人血小板中膜联蛋白的身份及其被诱导血小板脱颗粒的试剂释放的能力。在人血小板中检测到了与抗膜联蛋白I和V抗体发生交叉反应的蛋白质。然而，该研究提供的证据表明，这些膜联蛋白并非以Ca++依赖的方式位于质膜表面。此外，通过免疫印迹和酶联免疫吸附测定法确定，用几种诱导血小板脱颗粒的试剂激活血小板并不会导致膜联蛋白I或V的释放。
