Eldering J A, Kocher M, Clemetson J M, Clemetson K J, Frey F J, Frey B M
Department of Medicine, Inselspital, Berne, Switzerland.
FEBS Lett. 1993 Mar 8;318(3):231-4. doi: 10.1016/0014-5793(93)80518-y.
The present investigation revealed the presence of lipocortins I and IV, but not lipocortins II and VI, in human platelets. Lipocortin I was found in the Triton-soluble fraction of both resting and thrombin-activated platelets and was not covalently bound to skeletal components. Without detergents, when resting platelets were lysed and fractionated in the absence of Ca2+, lipocortin I was found only in the cytosolic fraction, whereas, in the presence of Ca2+, lipocortin I was associated only with the crude particulate and not with the membrane nor the cytosolic fractions.
目前的研究表明，人血小板中存在脂皮质素I和IV，但不存在脂皮质素II和VI。在静息血小板和凝血酶激活的血小板的Triton可溶性部分均发现了脂皮质素I，且它并非共价结合于骨架成分。在没有去污剂的情况下，当静息血小板在无Ca2+的条件下裂解并分级分离时，脂皮质素I仅存在于胞质部分；而在有Ca2+的情况下，脂皮质素I仅与粗颗粒部分相关，与膜部分和胞质部分均无关。
