Yoon H J, Park H J, Jhon G J
Department of Chemistry, College of Natural Science, Ewha Womans University, Seoul, Korea.
Biochem Int. 1992 Nov;28(3):393-9.
Effects of gangliosides in human erythrocyte membrane using carboxyl methyltransferase have been investigated. As GM3 concentration increased, methylatability for membrane was increased. But bovine brain ganglioside mixture did not alter methylatability even at a higher concentration. When 30 microM of GM3 was used in erythrocyte membrane, methylatability was increased by 32%. When purified-glucose transporters were reconstituted into egg phosphatidylcholine-liposome with GM3, methylatability was increased by 37%. Thus conformational difference of membrane according to localization of GM3 in intact human erythrocyte membrane or liposome possibly increases the methylatability and activity of glucose transporter itself.
已研究了使用羧甲基转移酶对人红细胞膜中神经节苷脂的作用。随着GM3浓度增加，膜的甲基化能力增强。但牛脑神经节苷脂混合物即使在较高浓度下也不会改变甲基化能力。当在红细胞膜中使用30微摩尔的GM3时，甲基化能力提高了32%。当将纯化的葡萄糖转运蛋白与GM3重构到鸡蛋磷脂酰胆碱脂质体中时，甲基化能力提高了37%。因此，根据GM3在完整人红细胞膜或脂质体中的定位，膜的构象差异可能会增加葡萄糖转运蛋白本身的甲基化能力和活性。
