David M J, Hellio M P, Portoukalian J, Richard M, Caton J, Vignon E
Department of Biochemistry, Edouard Herriot Hospital, Lyon, France.
J Rheumatol Suppl. 1995 Feb;43:133-5.
A decrease in all major gangliosides, contrasting with a marked increase in the GM3 third band (3B-GM3), has been demonstrated in osteoarthritic fibrillated cartilage. We found the 3B-GM3 in osteoarthritic fibrillated, grossly intact and osteophytic human cartilage, but it was undetectable in pig and bovine cartilage, bone, synovium, synovial fluid white cells, erythrocytes, and various extracellular tissues. We made a similar finding with lactosyl ceramide, the GM3 neutral glycolipid precursor. The search for an antibody against the 3B-GM3, as a possible specific antigenic marker of osteoarthritis (OA), was unsuccessful. However, an antibody against a molecule very close to 3B-GM3 on high performance thin layer chromatography, which will probably be identified as a glycosylphosphatidylinositol, was found in the serum of some patients with OA.
在骨关节炎的原纤维形成软骨中，已证实所有主要神经节苷脂减少，这与GM3第三带（3B - GM3）的显著增加形成对比。我们在骨关节炎的原纤维形成、大体完整和骨赘形成的人类软骨中发现了3B - GM3，但在猪和牛的软骨、骨骼、滑膜、滑膜液白细胞、红细胞以及各种细胞外组织中未检测到。我们在GM3中性糖脂前体乳糖基神经酰胺上也有类似发现。寻找针对3B - GM3的抗体作为骨关节炎（OA）可能的特异性抗原标志物未获成功。然而，在一些OA患者的血清中发现了一种针对在高效薄层色谱上与3B - GM3非常接近的分子的抗体，该分子可能会被鉴定为糖基磷脂酰肌醇。
