Linares A, Diaz R, Caamaño G J, Gonzalez F J, Garcia-Peregrin E
Departamento de Bioquímica y Biología Molecular, Universidad de Granada, Spain.
Biochem Int. 1992 Dec;28(4):683-91.
The effect of 24 h fasting on ketone body utilization by three extranervous tissues, liver, duodenum and kidney, was studied in two critical ages of neonatal chick: 4 and 9 days. In 4-day-old chick, plasma concentration of 3-hydroxybutyrate increased about 9-fold after 24 h starvation, while in 9-day-old chick this parameter increased about 23-fold in the same conditions. Hepatic lipogenesis from both precursors sharply decreased by fasting. Changes in the lipogenic activity of duodenum were less patent. However, we have found a clear increase in lipogenesis in chick kidney after 24 h starvation. CO2 production from acetoacetate was higher than that found from hydroxybutyrate. No significant differences in the acetoacetate oxidation to CO2 was observed in any tissue assayed after 24 h fasting. 14C incorporation from ketone bodies into amino acids was clearly decreased in kidney from 9-day-old chick by fasting. In liver and duodenum, acetoacetate incorporation into amino acids was higher than that from hydroxybutyrate.
在新生雏鸡的两个关键年龄阶段（4日龄和9日龄），研究了24小时禁食对肝脏、十二指肠和肾脏这三种非神经组织利用酮体的影响。在4日龄雏鸡中，饥饿24小时后，3-羟基丁酸的血浆浓度增加了约9倍，而在9日龄雏鸡中，在相同条件下该参数增加了约23倍。禁食使两种前体物质的肝脏脂肪生成急剧减少。十二指肠脂肪生成活性的变化不太明显。然而，我们发现饥饿24小时后雏鸡肾脏的脂肪生成明显增加。乙酰乙酸生成二氧化碳的量高于3-羟基丁酸。禁食24小时后，在所检测的任何组织中，乙酰乙酸氧化为二氧化碳均未观察到显著差异。禁食使9日龄雏鸡肾脏中酮体掺入氨基酸的14C明显减少。在肝脏和十二指肠中，乙酰乙酸掺入氨基酸的量高于3-羟基丁酸。
