PIRANI C L, STEPTO R C, SUTHERLAND K
J Exp Med. 1951 Mar;93(3):217-28. doi: 10.1084/jem.93.3.217.
The effect of desoxycorticosterone acetate (DCA) on the granulation tissue of healing and healed linear laparotomy wounds was studied in young adult male guinea pigs maintained on a complete diet and on a known intake of ascorbic acid. DCA induces the production of an excessive amount of granulation tissue, as evidenced by a relatively great number of fibroblasts and by a larger amount of ground substance. This effect was accompanied by a slight to moderate lag in the maturation process of both cellular and intercellular elements. These changes were observed when DCA administration was begun 5 days prior to operation, but were less obvious or absent if DCA was injected, beginning on the 5th or 10th postoperative day. The results indicate that the action of DCA on immature, proliferating connective tissue is marked, and is considerably less or absent when connective tissue elements have reached partial or almost complete maturity. The effect of DCA on connective tissue does not appear to rest on the basis of an altered nutritional status. Chemical and histochemical studies of the adrenals suggest that the action of DCA on connective tissue is probably mediated through a disturbance of adrenocortical function, namely an imbalance between hormones of the zona glomerulosa (excess of DCA) and those of the zona fasciculata (deficiency of glucocorticoids). The presence of changes in granulation tissue and the lack of them in mature resting connective tissue of DCA-treated guinea pigs confirm the view that a profound difference in the response mechanism exists between resting and actively proliferating connective tissue.
在给予完全饮食且已知抗坏血酸摄入量的成年雄性豚鼠身上，研究了醋酸脱氧皮质酮（DCA）对愈合中和已愈合的线性剖腹手术伤口肉芽组织的影响。DCA可诱导产生过量的肉芽组织，这表现为成纤维细胞数量相对较多以及基质含量较多。这种作用伴随着细胞和细胞间成分成熟过程中轻微至中度的延迟。当在手术前5天开始给予DCA时可观察到这些变化，但如果在术后第5天或第10天开始注射DCA，则这些变化不太明显或不存在。结果表明，DCA对未成熟的、增殖的结缔组织作用显著，而当结缔组织成分达到部分或几乎完全成熟时，其作用则明显减弱或不存在。DCA对结缔组织的作用似乎并非基于营养状况的改变。对肾上腺的化学和组织化学研究表明，DCA对结缔组织的作用可能是通过肾上腺皮质功能紊乱介导的，即球状带激素（DCA过量）和束状带激素（糖皮质激素缺乏）之间的失衡。在接受DCA治疗的豚鼠中，肉芽组织存在变化而成熟的静止结缔组织中没有变化，这证实了这样一种观点，即静止的和活跃增殖的结缔组织在反应机制上存在深刻差异。
