Salmon A M, Changeux J P
C.N.R.S. UA D1284 Neurobiologie Moléculairé, Institut Pasteur, Paris, France.
Neuroreport. 1992 Nov;3(11):973-6. doi: 10.1097/00001756-199211000-00006.
The role of motor innervation in the expression of beta-galactosidase targeted to the nucleus (nls beta gal) under the control of a chicken muscle acetylcholine receptor alpha-subunit promoter of 850 bp was investigated using two lines of transgenic mice. After birth, nls beta gal was transiently expressed in the endplate areas of extensor digitorum longus, tibialis anterior and diaphragm muscles. In the adult, denervation of several fast twitch muscles caused a burst of transgene expression which started at endplates and displayed defined though transient spatio-temporal patterns; in the slow soleus muscle, no regular patterns were observed. Thus, in vivo, the 850 bp promoter confers preferential expression of nls LacZ in the motor endplates of newborn mice and, in addition, directs expression of nls LacZ in denervated adult muscles.
利用两系转基因小鼠，研究了运动神经支配在850bp鸡肌肉乙酰胆碱受体α亚基启动子控制下靶向细胞核的β-半乳糖苷酶（nlsβgal）表达中的作用。出生后，nlsβgal在趾长伸肌、胫前肌和膈肌的终板区域短暂表达。在成年期，对几块快肌进行去神经支配会导致转基因表达爆发，这种爆发始于终板，并呈现出明确但短暂的时空模式；在慢肌比目鱼肌中，未观察到规律模式。因此，在体内，850bp启动子赋予新生小鼠运动终板中nls LacZ优先表达，此外，还指导nls LacZ在去神经支配的成年肌肉中表达。
