Gao F, Toriyama K, Nagata T
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Cell Mol Biol (Noisy-le-grand). 1992 Aug-Sep;38(5-6):661-8.
The DNA synthesis of mouse retina from the 19th prenatal day through 12 months postnatal has been studied by light microscopic radioautography after the injection of tritiated thymidine. A peak of the labeling index after incorporation of tritiated thymidine was found at fetal day 19. The labelled cells decreased gradually with the developing of the eye from the first postnatal day and were completely disappeared in two weeks after birth. The data also indicated obvious regional differences of the incorporation of tritiated thymidine during the periods of the retina development. The labeling index was the greatest in the anterior region compared to the equator region and the posterior region in the same group of age. The average number of the silver grains in labelled nucleus lead to a decrease with the development of the retina after birth, but there was no significant regional differences found in the same group of age. The data shown from this study suggest that the cell differentiation in mouse retina proceed from posterior to anterior region.
通过注射氚标记的胸腺嘧啶核苷后，利用光学显微镜放射自显影技术研究了小鼠从产前第19天到产后12个月视网膜的DNA合成情况。在胎儿第19天发现氚标记胸腺嘧啶核苷掺入后的标记指数峰值。从出生后第一天起，随着眼睛的发育，标记细胞逐渐减少，并在出生后两周内完全消失。数据还表明，在视网膜发育期间，氚标记胸腺嘧啶核苷的掺入存在明显的区域差异。在同一年龄组中，与赤道区和后区相比，前区的标记指数最大。出生后，随着视网膜的发育，标记细胞核中银颗粒的平均数量减少，但在同一年龄组中未发现明显的区域差异。本研究显示的数据表明，小鼠视网膜中的细胞分化从后区向前区进行。
