Gao F, Toriyama K, Ma H, Nagata T
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Cell Mol Biol (Noisy-le-grand). 1993 Jun;39(4):435-41.
The morphological change and DNA synthesis of the aging mice corneal cells were investigated by light microscopic radioautography after injection of tritiated thymidine. The result showed that the incorporation of tritiated thymidine in corneal cells changed with aging. The sites of tritiated thymidine incorporation were located in the epithelium from postnatal day 19 to 1 year after birth, in the stroma and endothelium from prenatal day 19 to postnatal day 8 only. The labeling index in the epithelial cells reached its highest value at 1 month after birth. Labelled stromal and endothelial cells reached their peaks simultaneously on the third day after birth and disappeared completely from postnatal 1 month onwards. The thickness of the cornea increased obviously at one month and there were no notable morphological change thereafter. Our investigation provides for the first time a systematic study on the age-related changes of DNA synthesis and construction in the aging mice corneas.
注射氚标记胸腺嘧啶核苷后，采用光学显微镜放射自显影法研究了衰老小鼠角膜细胞的形态变化和DNA合成。结果显示，角膜细胞中氚标记胸腺嘧啶核苷的掺入随衰老而变化。氚标记胸腺嘧啶核苷掺入部位在出生后19天至1岁时位于上皮细胞，仅在出生前19天至出生后8天位于基质和内皮细胞。上皮细胞中的标记指数在出生后1个月达到最高值。标记的基质和内皮细胞在出生后第3天同时达到峰值，并从出生后1个月起完全消失。角膜厚度在1个月时明显增加，此后无明显形态变化。我们的研究首次对衰老小鼠角膜中与年龄相关的DNA合成和结构变化进行了系统研究。
