Lehner C F, Ried G, Stern B, Knoblich J A
Friedrich-Miescher-Laboratorium Max-Planck-Gesellschaft, Tübingen, Germany.
Ciba Found Symp. 1992;170:97-109; discussion 110-4. doi: 10.1002/9780470514320.ch7.
Cyclin proteins and the kinases with which they associate are encoded by gene families in multicellular eukaryotes. A variety of cyclin/kinase complexes with different functions may exist. We have started a genetic dissection of this complexity in Drosophila. We have done experiments to investigate a potential functional overlap between two kinases (Dmcdc2 and Dmcdc2c) and two cyclins (cyclin A and cyclin B). No functional overlap was observed between the Dmcdc2 and the Dmcdc2c kinases. The phenotype resulting from mutations in Dmcdc2 was not affected by altering the level of Dmcdc2c. Our results concerning cyclin A and cyclin B strongly suggest that these two cyclins have largely overlapping functions. Cell proliferation was observed in the absence of either cyclin A or cyclin B, but not if both cyclins were absent. Cyclin A also has essential functions that cannot be taken over by cyclin B, but these functions appear to be required at defined developmental stages in specific tissues only.
细胞周期蛋白及其相关激酶由多细胞真核生物中的基因家族编码。可能存在多种具有不同功能的细胞周期蛋白/激酶复合物。我们已开始在果蝇中对这种复杂性进行遗传学剖析。我们进行了实验，以研究两种激酶（Dmcdc2和Dmcdc2c）与两种细胞周期蛋白（细胞周期蛋白A和细胞周期蛋白B）之间潜在的功能重叠。在Dmcdc2和Dmcdc2c激酶之间未观察到功能重叠。Dmcdc2突变产生的表型不受Dmcdc2c水平改变的影响。我们关于细胞周期蛋白A和细胞周期蛋白B的结果强烈表明，这两种细胞周期蛋白具有很大程度的重叠功能。在没有细胞周期蛋白A或细胞周期蛋白B的情况下观察到细胞增殖，但如果两种细胞周期蛋白都不存在则不会。细胞周期蛋白A也具有细胞周期蛋白B无法替代的重要功能，但这些功能似乎仅在特定组织的特定发育阶段才需要。
