臭氧对暴露于臭氧环境中的日本红点鲑（Salvelinus leucomaenis）红细胞中活性氧清除酶活性的影响。

Effect of ozone on the activities of reactive oxygen scavenging enzymes in RBC of ozone exposed Japanese charr (Salvelinus leucomaenis).

作者信息

Fukunaga K, Suzuki T, Hara A, Takama K

机构信息

Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, Hakodate, Japan.

出版信息

Free Radic Res Commun. 1992;17(5):327-33. doi: 10.3109/10715769209079525.

DOI:10.3109/10715769209079525

PMID:1483583

Abstract

The effect of ozone exposure on the activities of reactive oxygen scavenging enzymes (SOD, catalase, GSH-Px) in RBC of Japanese charr (Salvelinus leucomaenis) was examined. Ozone (0, 0.4 and 0.7 ppm as initial concentrations) was exposed to Japanese charr for 30 min, which definitely caused serious membrane damage to RBC of fish. Ozone exposure at 0.4 and 0.7 ppm decreased activities of both catalase and GSH-Px by 80 to 57% of the control. On the other hand, the activities of SOD remained unaffected even by 0.7 ppm ozone exposure. A hypothesis on the RBC membrane damage and participation of SOD and heme-iron was proposed.

摘要

研究了臭氧暴露对日本红点鲑（Salvelinus leucomaenis）红细胞中活性氧清除酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶）活性的影响。将臭氧（初始浓度分别为0、0.4和0.7 ppm）暴露于日本红点鲑30分钟，这无疑对鱼的红细胞造成了严重的膜损伤。暴露于0.4和0.7 ppm臭氧使过氧化氢酶和谷胱甘肽过氧化物酶的活性均降低至对照的80%至57%。另一方面，即使暴露于0.7 ppm的臭氧，超氧化物歧化酶的活性仍未受到影响。提出了关于红细胞膜损伤以及超氧化物歧化酶和血红素铁参与的假说。