Yaksh T L, Plant R L, Rudy T A
Eur J Pharmacol. 1977 Feb 21;41(4):399-408. doi: 10.1016/0014-2999(77)90260-6.
In rats, lesions were placed in the dorsal/median raphe (DMR), in the ventral raphe (VR: raphe magnus), in both the dorsal/median and ventral raphe (DMVR) or in the reticular formation (RF). The effect of the lesions on the antinociception and catalepsy produced by 3 doses of morphine (3, 10 and 30 mg/kg) was examined. The lesions had no significant effect on the catalepsy produced by any of the doses of morphine tested. DMR lesions produced a partial attentuation of the antinociceptive action of both the 3 and 10 mg doses. VR lesions produced a complete blockade of the 3 mg and only a partial attenuation of the 10 mg dose. In contrast, the combined (DMVR) lesions yielded virtually a total blockade of the 3 and 10 mg. Yet, as with the DMR and VR groups, the DMVR lesions failed to produce a significant antagonism on either of the nociceptive tests at the 30 mg dose. These findings suggest that the ascending and descending fiber systems emanating from the dorsal/median and ventral raphe, respectively, facilitate the expression of morphine-induced analgesia but that neither system alone can be regarded as essential for the manifestation of the antinociceptive effects of systematically administered morphine.
在大鼠中，损伤分别置于背侧/中缝核（DMR）、腹侧中缝核（VR：中缝大核）、背侧/中缝核与腹侧中缝核（DMVR）或网状结构（RF）。检测了这些损伤对3种剂量吗啡（3、10和30毫克/千克）产生的抗伤害感受和僵住症的影响。损伤对所测试的任何剂量吗啡产生的僵住症均无显著影响。DMR损伤使3毫克和10毫克剂量吗啡的抗伤害感受作用部分减弱。VR损伤使3毫克剂量吗啡的抗伤害感受作用完全被阻断，而对10毫克剂量仅部分减弱。相比之下，联合（DMVR）损伤使3毫克和10毫克剂量吗啡的抗伤害感受作用几乎完全被阻断。然而，与DMR和VR组一样，DMVR损伤在30毫克剂量时对任何一项伤害感受测试均未产生显著拮抗作用。这些发现表明，分别源自背侧/中缝核和腹侧中缝核的上行和下行纤维系统促进了吗啡诱导的镇痛作用的表达，但单独任何一个系统都不能被视为系统给予吗啡产生抗伤害感受作用所必需的。
